Welcome
to the Antibody Engineering Lab
Frederico Aires da Silva
to the Antibody Engineering Lab
Frederico Aires da Silva
André AS, Dias JNR, Aguiar S, Nogueira S, Bule P, Carvalho JI, António JPM, Cavaco M, Neves V, Oliveira S, Vicente G, Carrapiço B, Braz BS, Rütgen B, Gano L, Correia JDG, Castanho M, Goncalves J, Gois PMP, Gil S, Tavares L, Aires-da-Silva F. Rabbit derived VL single-domains as promising scaffolds to generate antibody-drug conjugates. Scientific Reports. 2023 Mar 24;13(1):4837. doi: 10.1038/s41598-023-31568-x.
André AS, Dias JNR, Aguiar SI, Leonardo A, Nogueira S, Amaral JD, Fernandes C, Gano L, Correia JDG, Cavaco M, et al. 2023. Panobinostat-loaded folate targeted liposomes as a promising drug delivery system for treatment of canine B-cell lymphoma. Frontiers in Veterinary Science. 10:1236136. doi:10.3389/fvets.2023.1236136.
Characterization of the canine CD20 as a therapeutic target for comparative passive immunotherapy. Sci Rep. 2022 Feb 17;12(1):2678. doi: 10.1038/s41598-022-06549-1
Aires-da-Silva F, Andre AS, Dias JNR. Aguiar SI, Oliveira S, Tavares L. Highly specific rabbit single-domain antibodies for drug delivery in immunotherapy applications. International Patent, PCT/IB2022/056303, 07 July 2022
André AS, Moutinho I, Dias JNR, Aires da Silva F. In vivo Phage Display: A promising selection strategy for the improvement of antibody targeting and drug delivery properties. Front Microbiol. 2022 Sep 26;13:962124. doi: 10.3389/fmicb.2022.962124..
Barroso MC, Grilo A, Aguiar S, Aires da Silva F, São Braz B. Occurrence of MDR1 1-delta mutation in herding dog breeds in Portugal. Front Vet Sci. 2022 Oct 3;9:990884. doi: 10.3389/fvets.2022.990884.
Istrate C, Marques J, Bule P, Correia S, Aires da Silva F, Duarte M, Reis AL, Machuqueiro M, Leitão A, Victor BL. In Silico Characterization of African Swine Fever Virus Nucleoprotein p10 Interaction with DNA. Viruses. 2022 Oct 25;14(11):2348. doi: 10.3390/v14112348.
Loss of α-gal during primate evolution enhanced antibody-effector function and resistance to bacterial sepsis. Cell Host Microbe. 2021 Mar 10;29(3):347-361.e12. doi: 10.1016/j.chom.2020.12.017. Epub 2021 Jan 25.PMID: 33497603
Ferreira M, Pinto S, Aires-da-Silva F, Bettencourt A, Aguiar S, Gaspar M. Liposomes as a Nanoplatform to Improve the Delivery of Antibiotics into Staphylococcus aureus Biofilms . Pharmaceutics 2021, 13(3), 321;02 Mar 2021.
Ferreira M, Ogren M, Dias JNR, Silva M, Gil S, Tavares L, Aires-da-Silva F, Gaspar MM, Aguiar SI. Liposomes as Antibiotic Delivery Systems: A Promising Nanotechnological Strategy against Antimicrobial Resistance. Molecules. 2021 Apr 2;26(7):2047. doi: 10.3390/molecules26072047.
Immunotherapeutic Strategies for Canine Lymphoma: Changing the Odds Against Non-Hodgkin Lymphoma. Front Vet Sci. 2021 Aug 26;8:621758. doi: 10.3389/fvets.2021.621758.
Chemokine-Directed Tumor Microenvironment Modulation in Cancer Immunotherapy. Int J Mol Sci. 2021 Sep 10;22(18):9804. doi: 10.3390/ijms22189804
Highly Specific Blood-Brain Barrier Transmigrating Single-Domain Antibodies Selected by an In Vivo Phage Display Screening. Pharmaceutics. 2021 Oct 2;13(10):1598. doi: 10.3390/pharmaceutics13101598
Diazaborines Are a Versatile Platform to Develop ROS-Responsive Antibody Drug Conjugates. Angew Chem Int Ed Engl. 2021 Nov 5. doi: 10.1002/anie.202109835.
Ministro JH, Oliveira SS, Oliveira JG, Cardoso M, Aires-da-Silva F, Corte-Real S, Goncalves J. Synthetic antibody discovery against native antigens by CRISPR/Cas9-library generation and endoplasmic reticulum screening. Appl Microbiol Biotechnol. 2020 Mar;104(6):2501-2512. doi: 10.1007/s00253-020-10423-3. Epub 2020 Feb 4.
Calado R, Duarte J, Borrego P, Marcelino JM, Bártolo I, Martin F, Figueiredo I, Almeida S, Graça L, Vítor J, Aires da Silva F, Dias I, Carrapiço B, Taveira N. A Prime-Boost Immunization Strategy with Vaccinia Virus Expressing Novel gp120 Envelope Glycoprotein from a CRF02_AG Isolate Elicits Cross-Clade Tier 2 HIV-1 Neutralizing Antibodies. Vaccines (Basel). 2020 Apr 7;8(2). pii: E171. doi: 10.3390/vaccines8020171.
Alves F, Prata S, Nunes T, Gomes J, Aguiar S, Aires da Silva F, Tavares L, Almeida V, Gil S. Canine parvovirus: a predicting canine model for sepsis. BMC Vet Res. 2020 Jun 15;16(1):199. doi: 10.1186/s12917-020-02417-0.
Aires da Silva F. Dias J. Aguiar J. Marques F. Andre A. Gil S. Tavares L. Novel Diagnostic and Therapeutic Immunologic Strategies to Overcome Infectious, Oncologic and Neurodegenerative Disorders. Advances in Animal Health, Medicine and Production pp 436-459. Springer
Dias JNR, Lopes M, Peleteiro C, Vicente G, Nunes T, Mateus L, Aires-da-Silva F, Tavares L, Gil S. Canine multicentric lymphoma exhibits systemic and intratumoral cytokine dysregulation. Vet Immunol Immunopathol. 2019 Dec;218:109940. doi: 10.1016/j.vetimm.2019.109940. Epub 2019 Sep 9.
Dias JNR, Andre A, Aguiar SA, Ministro J, Peleteiro MC, Rütgen B, Gano L, Correia JDG, Oliveira S, Goncalves J, Gil S, Tavares L, Aires-da-Silva F. Establishment of a bioluminescent canine B-cell lymphoma xenograft model for monitoring tumor progression and treatment response in preclinical studies. Plos One. (2018). Dec 28;13(12):e0208147. doi: 10.1371/journal.pone.0208147
Dias JNR, Aguiar SI, Pereira DM, André AS, Gano L, Correia JDG, Carrapiço B, Rütgen B, Malhó R, Peleteiro C, Goncalves J, Rodrigues CMP, Gil S, Tavares L, Aires-da-Silva F. The histone deacetylase inhibitor Panobinostat is a potent antitumor agent in canine diffuse large B-cell lymphoma. Oncotarget. (2018) Jun 19;9(47):28586-28598. doi: 10.18632/oncotarget.25580.
Aguiar S, Dias J, Manuel AM, Russo R, Gois PMP, Aires da Silva F, Goncalves J. Chimeric Small Antibody Fragments as Strategy to Deliver Therapeutic Payloads. Adv Protein Chem Struct Biol. 2018;112:143-182. doi: 10.1016/bs.apcsb.2018.03.002.
Goncalves J, Santos M, Acurcio R, Iria I, Gouveia L, Matos Brito P, Catarina Cunha-Santos A, Barbas A, Galvão J, Barbosa I, Aires da Silva F, Alcobia A, Cavaco M, Cardoso M, Delgado Alves J, Carey JJ, Dörner T, Eurico Fonseca J, Palmela C, Torres J, Lima Vieira C, Trabuco D, Fiorino G, Strik A, Yavzori M, Rosa I, Correia L, Magro F, D’Haens G, Ben-Horin S, Lakatos PL, Danese S. Antigenic response to CT-P13 and infliximab originator in inflammatory bowel disease patients shows similar epitope recognition. Aliment Pharmacol Ther. (2018) Sep;48(5):507-522. doi: 10.1111/apt.14808.
Gouveia Z, Carlos AR, Yuan X, Aires-da-Silva F, Stocker R, Maghzal GJ, Leal SS, Gomes CM, Todorovic S, Iranzo O, Ramos S, Santos AC, Hamza I, Gonçalves J, Soares MP. Characterization of plasma labile heme in hemolytic conditions. FEBS J. (2017) Oct;284(19):3278-3301. doi: 10.1111/febs.14192.
Neves V, Aires-da-Silva F, Morais M, Gano L, Ribeiro E, Pinto A, Aguiar S, Gaspar D, Fernandes C, Correia JD, Castanho MA. Novel Peptides Derived from Dengue Virus Capsid Protein Translocate Reversibly the Blood-Brain Barrier through a Receptor-Free Mechanism. ACS Chem Biol. (2017) May 19;12(5):1257-1268. doi: 10.1021/acschembio.7b00087.
Cantante C, Lourenço S, Morais M, Leandro J, Gano L, Silva N, Leandro P, Serrano M, Henriques AO, Andre A, Cunha-Santos C, Fontes C, Correia JDG, Aires da Silva F, Goncalves J. Albumin-binding domain from Streptococcus zooepidemicus protein Zag as a novel strategy to improve the half-life of therapeutic proteins. Journal of Biotechnology. 2017 Jul 10;253:23-33. 2017 Jul 10;253:23-33.
Neves V , Aires da Silva F, Corte-Real S, and Castanho M. Antibody Approaches To Treat Brain Diseases. Trends in Biotechnology. Vol. 34, No. 1. doi.org/10.1016/j.tibtech.2015.10.005.
Cunha-Santos C, Figueira TN, Borrego P, Oliveira SS, Rocha C, Couto A, Cantante C, Santos-Costa Q, Azevedo-Pereira JM, Fontes CM, Taveira N, Aires-Da-Silva F, Castanho MA, Veiga AS, Goncalves J. Development of synthetic light-chain antibodies as novel and potent HIV fusion inhibitors. AIDS. (2016) Jul 17;30(11):1691-701. doi: 10.1097/QAD.0000000000001108.
Morais M, Cantante C, Gano L, Santos I, Lourenço S, Santos C, Fontes C, Aires da Silva F, Gonçalves J, Correia JD. Biodistribution of a (67)Ga-labeled anti-TNF VHH single-domain antibody containing a bacterial albumin-binding domain (Zag). Nucl Med Biol. (2014) May;41 Suppl:e44-8. doi: 10.1016/j.nucmedbio.2014.01.009.
Aires da Silva F, Li M, Rato S, Maia S, Malhó R, Craigie R, Barbas III C, Joao Goncalves. Recombinant rabbit single-chain antibodies bind to the catalytic and C-terminal domains of HIV-1 integrase protein and strongly inhibit HIV-1 infection. Biotechnology and Applied Biochemistry. (2012) Sep-Oct;59(5):353-66. doi: 10.1002/bab.1034.
Oliveira S, Aires da Silva F, Lourenco S, Freitas-Vieira A, Cunha Santos A, Goncalves J. Assessing combinatorial strategies to multimerize libraries of single-domain antibodies. Biotechnology and Applied Biochemistry. (2012) May-Jun;59(3):193-204. doi: 10.1002/bab.1011.
Corte-Real S, Aires da Silva F, & Gonçalves J. Development of Antibody Fragments for Therapeutic Applications. Peptide Drug Discovery and Development: Translational Research in Academia and Industry, First Edition. Chapter 16; 337-354. Published 2011 by WILEY-VCH Verlag GmbH & Co. KgaA. doi.org/10.1002/9783527636730.ch16
Aires da Silva F, Corte-Real S, Goncalves J. Recombinant Antibodies as Therapeutic Agents: Pathways for Modelling New Biodrugs. Biodrugs. (2008) ;22(5):301-14. Review. doi:10.2165/00063030-200822050-00003.
Ferreira GN, Encarnação JM, Rosa L, Rodrigues R, Breyner R, Barrento S, Pedro L, Aires da Silva F, Gonçalves J. Recombinant single-chain variable fragment and single domain antibody piezoimmunosensors for detection of HIV1 virion infectivity factor.
Biosens Bioelectron. (2007) Oct 31;23(3):384-92. doi.org/10.1016/j.bios.2007.04.022.
Encarnação JM, Rosa L, Rodrigues R, Pedro L, da Silva FA, Gonçalves J, Ferreira GN. Piezoelectric biosensors for biorecognition analysis: application to the kinetic study of HIV-1 Vif protein binding to recombinant antibodies. J Biotechnol. (2007) Oct 31;132(2):142-8. doi.org/10.1016/j.jbiotec.2007.04.010.
Santa-Marta M, Aires da Silva F, Fonseca AM, Rato S, Goncalves J. HIV-1 Vif protein blocks the cytidine deaminase activity of B-cell specific AID in E. coli by a similar mechanism of action. Mol Immunol. (2007) Jan;44(4):583-90. doi.org/10.1016/j.molimm.2006.02.005
Aires da Silva F, Costa MJ, Corte-Real S, Goncalves J. Cell type-specific targeting with Sindbis pseudotyped lentiviral vectors displaying anti-CCR5 single-chain antibodies. Human Gene Therapy. (2005), 16:223-234. doi.org/10.1089/hum.2005.16.223.
Corte-Real S, Collins C, Aires da Silva F, Simas P, Barbas C, Chang Y. Moore P. Goncalves J. Intrabodies targeting the Kaposi’s sarcoma-associated herpesvirus latency antigen inhibit viral persistence in lymphoma cells. Blood. (2005),106:3797-3802. doi.org/10.1182/blood-2005-04-1627.
Santa-Marta M, Aires da Silva F, Fonseca AM, Goncalves J. HIV-1 Vif can directly inhibit APOBEC3G-mediated cytidine deamination by using a single amino acid interaction and without protein degradation. J. Biol Chem. (2005), 280:8765-75. doi.org/10.1016/j.molimm.2006.02.005.
Aires da Silva F, Santa-Marta F, Freitas-Vieira A, Mascarenhas P, Barahona I, Moniz-Pereira J, Goncalves J. ‘Camelizing’ Rabbit VH Single-Domain Intrabodies Against Vif Protein Strongly Neutralize HIV-1 Infectivity. J. Mol Biol. (2004), 340, 525-542. doi.org/10.1016/j.jmb.2004.04.062.
Goncalves J, Aires da Silva F, Freitas-Vieira A, Santa-Marta M, Malhó R, Yang X, Gabuzda D, Barbas CF III. Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication. J. Biol. Chem. (2002), 277: 32036-32045.